ANPEP CLIA Kit for Human(Homo sapiens) | EIAab.comANPEP CLIA Kit for Human(Homo sapiens)

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ANPEP (Gene name),

Aminopeptidase N (Protein name ), AMPN_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

ANPEP(APN;CD13;PEPN);

Protein name:

Aminopeptidase N(AP-N;hAPN);

Alternative:

Aminopeptidase M(AP-M);Alanyl aminopeptidase;Myeloid plasma membrane glycoprotein CD13;Microsomal aminopeptidase;gp150;

CD:

CD13;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Homodimer. Interacts with the S1 domain of HCoV-229E spike protein.

Function:

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.

Subcellular Location:

Cell membrane Single-pass type II membrane protein Cytoplasm cytosol A soluble form has also been detected.

Protein Attributes:

Sequence length:

967

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

900:

950:

967:

VKENKEVVLQ | WFTENSK

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

P15144

KEGG:

hsa:290

UniGene:

Hs.1239

SMR:

P15144

MIM:

151530

Pfam:

PF01433

STRING:

9606.ENSP00000300060

Pfam:

PF11838

Uniprot:

P15144

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ELISA Kit for Human AP-N

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E9154m

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ELISA Kit for Human AP-N

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E9154h

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ELISA Kit for Human AP-N

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E9154p

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ELISA Kit for Human AP-N

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E9154c

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ELISA Kit for Human AP-N

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E9154r

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ELISA Kit for Human AP-N

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ELISA Kit for Human AP-N

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E9154d

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96T

ELISA Kit for Human AP-N

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E9154b

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96T

CLIA Kit for Human AP-N

Cat.:

U9154p

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96T

CLIA Kit for Human AP-N

Cat.:

U9154h

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96T

CLIA Kit for Human AP-N

Cat.:

U9154m

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96T

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CLIA Kit for Human AP-N

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CLIA Kit for Human AP-N

Cat.:

U9154d

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96T

CLIA Kit for Human AP-N

Cat.:

U9154r

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96T

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CLIA Kit for Human AP-N

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U9154b

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96T

CLIA Kit for Human AP-N

Cat.:

U9154c

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Polyclonal Antibody for Human AP-N

Cat.:

P9154Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human AP-N

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P9154Rb-h

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Polyclonal Antibody for Human AP-N

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P9154Rb-m

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Monoclonal Antibody for Human AP-N

Protein for Human AP-N

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Complete amino acid sequence of human intestinal aminopeptidase N as deduced from cloned cDNA."
Olsen J. , Cowell G.M. , Koenigshoefer E. , Danielsen E.M. , Moeller J. , Laustsen L. , Hansen O.C. , Welinder K.G. , Engberg J. , Hunziker W. , Spiess M. , Sjoestroem H. , Noren O.
FEBS Lett.238:307-314(1988) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];VARIANTS GLN-86 AND MET-603
tissue: Intestine.
2.

"Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N."
Look A.T. , Ashmun R.A. , Shapiro L.H. , Peiper S.C.
J. Clin. Invest.83:1299-1307(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];PROTEIN SEQUENCE OF 2-21;VARIANT GLN-86
3.

"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C. , Garber M. , Sharpe T. , Young S.K. , Rowen L. , O'Neill K. , Whittaker C.A. , Kamal M. , Chang J.L. , Cuomo C.A. , Dewar K. , FitzGerald M.G. , Kodira C.D. , Madan A. , Qin S. , Yang X. , Abbasi N. , Abouelleil A. , more...
Nature440:671-675(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
4.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Pancreas.
5.

"Separate promoters control transcription of the human aminopeptidase N gene in myeloid and intestinal epithelial cells."
Shapiro L.H. , Ashmun R.A. , Roberts W.M. , Look A.T.
J. Biol. Chem.266:11999-12007(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15
tissue: Intestinal epithelium.
6.

"Genomic organisation of aminopeptidase N."
Eiz-Vesper B. , Fuchs N. , Gottschalk D. , Mueller K. , Reuter S. , Blasczyk R.
Submitted (2002-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-479 AND 524-967;VARIANT GLN-86
tissue: Peripheral blood.
7.

"Identification of an alanine aminopeptidase in human maternal serum as a membrane-bound aminopeptidase N."
Watanabe Y. , Iwaki-Egawa S. , Mizukoshi H. , Fujimoto Y.
Biol. Chem. Hoppe-Seyler376:397-400(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-20 AND 70-81
8.

"Cholesterol crystallization-promoting activity of aminopeptidase-N isolated from the vesicular carrier of biliary lipids."
Nunez L. , Amigo L. , Rigotti A. , Puglielli L. , Mingrone G. , Greco A.V. , Nervi F.
FEBS Lett.329:84-88(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-18
9.

"Purification and characterization of aminopeptidase N from human plasma."
Tokioka-Terao M. , Hiwada K. , Kokubu T.
Enzyme32:65-75(1984) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION;SUBUNIT
10.

"Variable O-glycosylation of CD13 (aminopeptidase N)."
O'Connell P.J. , Gerkis V. , d'Apice A.J.F.
J. Biol. Chem.266:4593-4597(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION
11.

"Human aminopeptidase N is a receptor for human coronavirus 229E."
Yeager C.L. , Ashmun R.A. , Williams R.K. , Cardellichio C.B. , Shapiro L.H. , Look A.T. , Holmes K.V.
Nature357:420-422(1992) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HCOV-229E SPIKE GLYCOPROTEIN;CHARACTERIZATION OF HCOV-229E RECEPTOR FUNCTION
12.

"CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated."
Favaloro E.J. , Browning T. , Facey D.
Exp. Hematol.21:1695-1701(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF SOLUBLE FORM
13.

"CD13 (human aminopeptidase N) mediates human cytomegalovirus infection."
Soderberg C. , Giugni T.D. , Zaia J.A. , Larsson S. , Wahlberg J.M. , Moller E.
J. Virol.67:6576-6585(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CHARACTERIZATION OF CMV RECEPTOR FUNCTION
14.

"Characterization of functional domains in the human coronavirus HCV 229E receptor."
Kolb A.F. , Maile J. , Heister A. , Siddell S.G.
J. Gen. Virol.77:2515-2521(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR HCOV-229E INFECTION
15.

"Defectively N-glycosylated and non-O-glycosylated aminopeptidase N (CD13) is normally expressed at the cell surface and has full enzymatic activity."
Noren K. , Hansen G.H. , Clausen H. , Noren O. , Sjostrom H. , Vogel L.K.
Exp. Cell Res.231:112-118(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;GLYCOSYLATION
16.

"Identification of residues critical for the human coronavirus 229E receptor function of human aminopeptidase N."
Kolb A.F. , Hegyi A. , Siddell S.G.
J. Gen. Virol.78:2795-2802(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF RESIDUES CRITICAL FOR HCOV-229E INFECTION
17.

"Characterization of determinants involved in the feline infectious peritonitis virus receptor function of feline aminopeptidase N."
Hegyi A. , Kolb A.F.
J. Gen. Virol.79:1387-1391(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR TGEV INFECTION
18.

"Modification of the amino terminus of a class II epitope confers resistance to degradation by CD13 on dendritic cells and enhances presentation to T cells."
Dong X. , An B. , Salvucci Kierstead L. , Storkus W.J. , Amoscato A.A. , Salter R.D.
J. Immunol.164:129-135(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;ENZYMATIC CLEAVAGE OF ANTIGEN PEPTIDES BOUND TO CLASS II MHC
19.

"Aminopeptidase N is a receptor for tumor-homing peptides and a target for inhibiting angiogenesis."
Pasqualini R. , Koivunen E. , Kain R. , Lahdenranta J. , Sakamoto M. , Stryhn A. , Ashmun R.A. , Shapiro L.H. , Arap W. , Ruoslahti E.
Cancer Res.60:722-727(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ROLE IN ANGIOGENESIS;CHARACTERIZATION OF RECEPTOR FOR TUMOR-HOMING PEPTIDES FUNCTION
20.

"Expression of aminopeptidase N in human endometrium and regulation of its activity by estrogen."
Seli E. , Senturk L.M. , Bahtiyar O.M. , Kayisli U.A. , Arici A.
Fertil. Steril.75:1172-1176(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INDUCTION BY ESTRADIOL AND IL8
21.

"Molecular determinants of species specificity in the coronavirus receptor aminopeptidase N (CD13): influence of N-linked glycosylation."
Wentworth D.E. , Holmes K.V.
J. Virol.75:9741-9752(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF 288-ASP--SER-295 AND ASN-818
22.

"Soluble aminopeptidase N/CD13 in malignant and nonmalignant effusions and intratumoral fluid."
van Hensbergen Y. , Broxterman H.J. , Hanemaaijer R. , Jorna A.S. , van Lent N.A. , Verheul H.M. , Pinedo H.M. , Hoekman K.
Clin. Cancer Res.8:3747-3754(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION OF SOLUBLE FORM
23.

"Identification of a receptor-binding domain of the spike glycoprotein of human coronavirus HCoV-229E."
Bonavia A. , Zelus B.D. , Wentworth D.E. , Talbot P.J. , Holmes K.V.
J. Virol.77:2530-2538(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH HCOV-229E SPIKE GLYCOPROTEIN
24.

"A proteomic analysis of human bile."
Kristiansen T.Z. , Bunkenborg J. , Gronborg M. , Molina H. , Thuluvath P.J. , Argani P. , Goggins M.G. , Maitra A. , Pandey A.
Mol. Cell. Proteomics3:715-728(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-265
tissue: Bile.
25.

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T. , Qian W.-J. , Gritsenko M.A. , Camp D.G. II , Monroe M.E. , Moore R.J. , Smith R.D.
J. Proteome Res.4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-128; ASN-234; ASN-265; ASN-681 AND ASN-818
tissue: Plasma.
26.

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R. , Jiang X. , Sun D. , Han G. , Wang F. , Ye M. , Wang L. , Zou H.
J. Proteome Res.8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-128; ASN-234; ASN-265; ASN-573; ASN-681 AND ASN-818
tissue: Liver.
27.

"Initial characterization of the human central proteome."
Burkard T.R. , Planyavsky M. , Kaupe I. , Breitwieser F.P. , Buerckstuemmer T. , Bennett K.L. , Superti-Furga G. , Colinge J.
BMC Syst. Biol.5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
28.

"The X-ray crystal structure of human aminopeptidase N reveals a novel dimer and the basis for peptide processing."
Wong A.H. , Zhou D. , Rini J.M.
J. Biol. Chem.287:36804-36813(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 66-967 IN COMPLEX WITH ANGIOTENSIN-4 AND INHIBITORS;CATALYTIC ACTIVITY;COFACTOR;SUBSTRATE-BINDING REGION;ZINC-BINDING SITES;ACTIVE SITE;GLYCOSYLATION AT ASN-128; ASN-234; ASN-265; ASN-319; ASN-527; ASN-625; ASN-681 AND ASN-818;SUBUNIT;DISULFIDE BONDS
29.

"Identification of point mutations in the aminopeptidase N gene by SSCP analysis and sequencing."
Lendeckel U. , Wex T. , Arndt M. , Frank K. , Franke A. , Ansorge S.
Hum. Mutat. Suppl.1:S158-S160(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS TYR-242 AND PRO-243

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